Differential effects of 9-cis and all-trans retinoic acid on the induction of retinoic acid receptor-β and cellular retinoic acid-binding protein II in human neuroblastoma cells

Novel retinoic acid, 9-cis retinoic acid, in combination with all-trans retinoic acid is an effective inducer of differentiation of retinoic acid-resistant HL-60 cells.

Recent studies have shown that a high proportion of patients with acute promyelocytic leukemia (APL) achieve complete remission after treatment with all-trans retinoic acid (RA). Nevertheless, despite an initial good response, most patients that received continuous treatment with all-trans RA relapse and develop RA-resistant disease. The 9-cis RA is a high-affinity ligand for retinoid X recepto...

Effects of Novel Retinoic Acid Compound, 9-cis-Retinoic Acid, on Proliferation, Differentiation, and Expression of Retinoic Acid Receptor-a

Retinoic acid modulates proliferation and differentiation of a wide variety of normal and leukemic cells through two distinct families of transcriptional factors: the retinoic acid receptors (RARs) and the retinoid X receptors (RXRs). A stereoisomer of retinoic acid, 9-cis-retinoic acid, is a highaffinity ligand for RXR and binds efficiently to RAR. In contrast, all-trans-retinoic acid interact...

Isomerization of all-trans-retinoic acid to 9-cis-retinoic acid.

The discovery of the biological activity of 9-cis-retinoic acid raises questions as to its mode of biosynthesis. A simple mechanism involves the direct isomerization of all-trans-retinoic acid to 9-cis-retinoic acid. It is shown here that bovine liver membranes, but not supernatant fractions, can isomerize all-trans-retinoic acid into 9-cis-retinoic acid and 13-cis-retinoic acid. The concentrat...

Direct channeling of retinoic acid between cellular retinoic acid-binding protein II and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest.

Cellular retinoic acid-binding protein II (CRABP-II) is an intracellular lipid-binding protein that associates with retinoic acid with a subnanomolar affinity. We previously showed that CRABP-II enhances the transcriptional activity of the nuclear receptor with which it shares a common ligand, namely, the retinoic acid receptor (RAR), and we suggested that it may act by delivering retinoic acid...

Acid-Induced Growth Arrest Mammary Carcinoma Cells to Retinoic Retinoic Acid Receptor Sensitizes and Cellular Retinoic Acid-Binding Protein II Direct Channeling of Retinoic Acid between